[PDF][PDF] WHIM Syndrome With a Novel CXCR4 Variant in a Korean Child

QY Wang, HR Zhang, Y Gao, RH Li… - Annals of laboratory …, 2017 - synapse.koreamed.org
QY Wang, HR Zhang, Y Gao, RH Li, XH Shang, DW Shin, SN Park, SM Kim, K Im, JA Kim…
Annals of laboratory medicine, 2017synapse.koreamed.org
Dear Editor, Persistent infection with human papillomavirus (HPV) is the main cause of
cervical cancer. Screening of cervical specimens for high-risk human papillomaviruses (HR-
HPV) has become a routine part of patient care in most countries. The Roche cobas 4800
system (Roche Molecular Systems Inc., Pleasanton, CA, USA), a fully automated system that
uses in vitro qualitative realtime PCR technology, displays high sensitivity and specificity for
HR-HPV detection [1]. However, a small proportion of invalid results may occur in specimens …
Dear Editor, Persistent infection with human papillomavirus (HPV) is the main cause of cervical cancer. Screening of cervical specimens for high-risk human papillomaviruses (HR-HPV) has become a routine part of patient care in most countries. The Roche cobas 4800 system (Roche Molecular Systems Inc., Pleasanton, CA, USA), a fully automated system that uses in vitro qualitative realtime PCR technology, displays high sensitivity and specificity for HR-HPV detection [1]. However, a small proportion of invalid results may occur in specimens showing mucopurulent discharge because of pipetting errors, clot formation, or failed amplification, which could cause a delay in the results and lead to additional waiting time for patients and extra work hours for staff, although most cases are resolved by vortexing the specimens and retesting. To the best of our knowledge, no researcher has tried to overcome this problem by pretreating specimens with an appropriate concentration of Sputasol (Oxoid Ltd., Basingstoke, United Kingdom); Sputasol (dithiothreitol 1.4%) has been used to aid culturing and DNA extraction from sputum specimens in some laboratories [2]. Here, we propose a standard method to improve HR-HPV detection without affecting the performance of the cobas 4800 HPV system.
synapse.koreamed.org