Metastasis is the main cause of cancer-related deaths and remains the most significant
challenge to management of the disease. Metastases are established through a complex
multistep process involving intracellular signaling pathways. To gain insight to proteins
central to specific steps in metastasis formation, we used a metastasis cell line model that
allows investigation of extravasation and colonization of circulating cancer cells to lungs in
mice. Using stable isotopic labeling by amino acids in cell culture and subcellular …