A novel chemistry for conjugating pneumococcal polysaccharides to Luminex microspheres
SA Schlottmann, N Jain, N Chirmule… - Journal of immunological …, 2006 - Elsevier
Here we describe a novel method to conjugate pneumococcal polysaccharides (PnPS) to
Luminex microspheres for use in serological assays. 4-(4, 6-dimethoxy [1, 3, 5] triazin-2-yl)-4-
methyl-morpholinium (DMTMM) modification of PnPS and conjugation to carboxyl functional
groups on Luminex microspheres (COOH–DMTMM method) was shown to be a
reproducible chemistry that efficiently conjugated PnPS to Luminex microspheres without
affecting the antigenicity of a broad set of PnPS. The COOH–DMTMM method was …
Luminex microspheres for use in serological assays. 4-(4, 6-dimethoxy [1, 3, 5] triazin-2-yl)-4-
methyl-morpholinium (DMTMM) modification of PnPS and conjugation to carboxyl functional
groups on Luminex microspheres (COOH–DMTMM method) was shown to be a
reproducible chemistry that efficiently conjugated PnPS to Luminex microspheres without
affecting the antigenicity of a broad set of PnPS. The COOH–DMTMM method was …