[HTML][HTML] Dissecting the antibody-OME: past, present, and future
C Loos, DA Lauffenburger, G Alter - Current Opinion in Immunology, 2020 - Elsevier
C Loos, DA Lauffenburger, G Alter
Current Opinion in Immunology, 2020•ElsevierHighlights•Humoral immunity is mediated by complex networks of polyclonal antibodies
interacting with antigens and effector immune cells.•This network integrates properties of the
Fab and Fc domains of antibodies, governed by regulatory processes within B-cells.•Current
OMIC tools capture individual aspects of antibody Fab, Fc, and transcriptional B-cell
properties separately.•Computational frameworks are required to integrate, as
comprehensively as possible, the complexity of humoral immunity.Humoral immunity is key …
interacting with antigens and effector immune cells.•This network integrates properties of the
Fab and Fc domains of antibodies, governed by regulatory processes within B-cells.•Current
OMIC tools capture individual aspects of antibody Fab, Fc, and transcriptional B-cell
properties separately.•Computational frameworks are required to integrate, as
comprehensively as possible, the complexity of humoral immunity.Humoral immunity is key …
Highlights
- Humoral immunity is mediated by complex networks of polyclonal antibodies interacting with antigens and effector immune cells.
- This network integrates properties of the Fab and Fc domains of antibodies, governed by regulatory processes within B-cells.
- Current OMIC tools capture individual aspects of antibody Fab, Fc, and transcriptional B-cell properties separately.
- Computational frameworks are required to integrate, as comprehensively as possible, the complexity of humoral immunity.
Humoral immunity is key to protection for nearly all licensed vaccines. Yet, the design of vaccines has been more difficult for some of our most deadly killers (eg HIV, influenza, Dengue virus, etc.), likely due to our incomplete understanding of the precise immunological mechanisms associated with protection. Humoral immunity is governed both by B-cells and their bi-functional secreted antibodies, all of which have a unique capacity to evolve during an immune response. Current OMIC technologies capture individual features of the humoral immune response, providing a glimpse into humoral components (Fab/Fc/B-cell-omic), but fail to provide a wholistic view of the humoral response as a collective functional arm. Here, we dissect current OMIC strategies reviewing experimental and computational approaches, that if integrated could provide a true systems-level view of the humoral immune response.
Elsevier