Gamma/delta T cell clones and natural killer cell clones mediate distinct patterns of non-major histocompatibility complex-restricted cytolysis.
P Fisch, M Malkovsky, E Braakman, E Sturm… - Journal of Experimental …, 1990 - rupress.org
P Fisch, M Malkovsky, E Braakman, E Sturm, RL Bolhuis, A Prieve, JA Sosman, VA Lam…
Journal of Experimental Medicine, 1990•rupress.orgMaterials and Methods Cytotoxic Clones. PBMC were isolated by Ficoll-Hypaque
sedimentation of heparinized blood samples; most experiments described involve PBL
obtained from one healthy volunteer blood donor. These were positively selected by FAGS
for cells expressing the Leu-19 antigen (predominantly NK cells), the CD16 (Leu-11a)
antigen (NK cells), or y/b T cells. The sorted cells were cloned by limiting dilution at 0.3 cells
per well (5, 6, 15). The culture medium
sedimentation of heparinized blood samples; most experiments described involve PBL
obtained from one healthy volunteer blood donor. These were positively selected by FAGS
for cells expressing the Leu-19 antigen (predominantly NK cells), the CD16 (Leu-11a)
antigen (NK cells), or y/b T cells. The sorted cells were cloned by limiting dilution at 0.3 cells
per well (5, 6, 15). The culture medium
Materials and Methods
Cytotoxic Clones. PBMC were isolated by Ficoll-Hypaque sedimentation of heparinized blood samples; most experiments described involve PBL obtained from one healthy volunteer blood donor. These were positively selected by FAGS for cells expressing the Leu-19 antigen (predominantly NK cells), the CD16 (Leu-11a) antigen (NK cells), or y/b T cells. The sorted cells were cloned by limiting dilution at 0.3 cells per well (5, 6, 15). The culture medium
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