Accurate Measurement of Nicotinamide Adenine Dinucleotide (NAD+) with High-Performance Liquid Chromatography
J Yoshino, S Imai - Sirtuins: Methods and protocols, 2013 - Springer
J Yoshino, S Imai
Sirtuins: Methods and protocols, 2013•SpringerNicotinamide adenine dinucleotide (NAD+) plays a critical role in regulating numerous
biological and physiological pathways including metabolism, inflammation, cancer, and
aging in mammals. Here we describe a highly quantitative method with reverse-phase high-
performance liquid chromatography (HPLC) for the determination of NAD+ levels in cells
and tissues. This methodology provides accurate, reliable, and reproducible results of NAD+
measurement, which enables us to analyze various pathophysiological changes in NAD+ …
biological and physiological pathways including metabolism, inflammation, cancer, and
aging in mammals. Here we describe a highly quantitative method with reverse-phase high-
performance liquid chromatography (HPLC) for the determination of NAD+ levels in cells
and tissues. This methodology provides accurate, reliable, and reproducible results of NAD+
measurement, which enables us to analyze various pathophysiological changes in NAD+ …
Abstract
Nicotinamide adenine dinucleotide (NAD+) plays a critical role in regulating numerous biological and physiological pathways including metabolism, inflammation, cancer, and aging in mammals. Here we describe a highly quantitative method with reverse-phase high-performance liquid chromatography (HPLC) for the determination of NAD+ levels in cells and tissues. This methodology provides accurate, reliable, and reproducible results of NAD+ measurement, which enables us to analyze various pathophysiological changes in NAD+ levels in vitro and in vivo.
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