Increased frequency of intracellular interleukin (IL)‐13 and IL‐10, but not IL‐4, expressing CD4+ and CD8+ peripheral T cells of patients with atopic dermatitis
M Aleksza, B Irinyi, A Lukacs… - British Journal of …, 2002 - academic.oup.com
British Journal of Dermatology, 2002•academic.oup.com
Background A number of studies exist demonstrating the increased expression of type 2
cytokines and decreased capacity to produce interferon‐γ (IFN‐γ) in peripheral blood
mononuclear cells (PBMCs) of patients with atopic dermatitis (AD). Objectives To clarify the
results of recent studies concerning the role of interleukin (IL)‐4 and IL‐13 in PBMCs of AD
patients, we analysed the activation status of lymphocyte subpopulations. Methods We
measured the intracellular expression and serum levels of certain type 1 and type 2 …
cytokines and decreased capacity to produce interferon‐γ (IFN‐γ) in peripheral blood
mononuclear cells (PBMCs) of patients with atopic dermatitis (AD). Objectives To clarify the
results of recent studies concerning the role of interleukin (IL)‐4 and IL‐13 in PBMCs of AD
patients, we analysed the activation status of lymphocyte subpopulations. Methods We
measured the intracellular expression and serum levels of certain type 1 and type 2 …
Abstract
Summary Background A number of studies exist demonstrating the increased expression of type 2 cytokines and decreased capacity to produce interferon‐γ (IFN‐γ) in peripheral blood mononuclear cells (PBMCs) of patients with atopic dermatitis (AD).
Objectives To clarify the results of recent studies concerning the role of interleukin (IL)‐4 and IL‐13 in PBMCs of AD patients, we analysed the activation status of lymphocyte subpopulations.
Methods We measured the intracellular expression and serum levels of certain type 1 and type 2 cytokines, using cell surface and intracellular cytokine staining, flow cytometry and enzyme‐linked immunosorbent assay techniques.
Results The frequency of IL‐10 and IL‐13 producing CD4+ and CD8+ T cells was significantly higher in patients with AD, while the frequency of IFN‐γ secreting helper and cytotoxic T cells was significantly lower in patients with AD than in control subjects. The serum levels of IL‐10 and IL‐13 were also significantly increased. There were no significant differences observed between the experimental groups in the frequency of IL‐4 producing CD4+ and CD8+ cells.
Conclusions This study demonstrates a type 2 cytokine production in the CD4+ and CD8+ T cells of AD patients, which is characterized by an elevated IL‐13, but not by IL‐4 secretion, and by an increased level of the immunoregulatory IL‐10, which can contribute to a decrease in IFN‐γ expression.
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