Observations related to the pathogenesis of dengue hemorrhagic fever. II. Antigenic and biologic properties of dengue viruses and their association with disease …
SB Halstead, P Simasthien - The Yale journal of biology and …, 1970 - ncbi.nlm.nih.gov
SB Halstead, P Simasthien
The Yale journal of biology and medicine, 1970•ncbi.nlm.nih.govMATERIALS AND METHODS Plaque reduction test The production of LLC-MK2
monolayers, the techniques of plaque assay and the plaque reduction neutralization test
have been givenelsewhere.'In all tests, six repli-cate 2 oz. plaque bottles were used. All
neutralization tests reported in this paper were performed by incubating serum and virus
mixtures at 22-250C. for exactly 60 minutes. Reactions were stopped by dilution of serum-
virus mixtures to 1: 100 in chilled 0.75% bovine albumin-phosphate buffered saline, pH 7.8 …
monolayers, the techniques of plaque assay and the plaque reduction neutralization test
have been givenelsewhere.'In all tests, six repli-cate 2 oz. plaque bottles were used. All
neutralization tests reported in this paper were performed by incubating serum and virus
mixtures at 22-250C. for exactly 60 minutes. Reactions were stopped by dilution of serum-
virus mixtures to 1: 100 in chilled 0.75% bovine albumin-phosphate buffered saline, pH 7.8 …
MATERIALS AND METHODS Plaque reduction test The production of LLC-MK2 monolayers, the techniques of plaque assay and the plaque reduction neutralization test have been givenelsewhere.'In all tests, six repli-cate 2 oz. plaque bottles were used. All neutralization tests reported in this paper were performed by incubating serum and virus mixtures at 22-250C. for exactly 60 minutes. Reactions were stopped by dilution of serum-virus mixtures to 1: 100 in chilled 0.75% bovine albumin-phosphate buffered saline, pH 7.8-8.0 (BAPS) or 20% heat inactivated agamma calf serum in phosphatebuffered saline, pH 7.8-8.9. Residual virus was incubated on cell sheets for 90 minutes at 370C. before agar overlay.
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