Isolation of malignant B cells from patients with chronic lymphocytic leukemia (CLL) for analysis of cell proliferation: validation of a simplified method suitable for multi …
GM Hayes, R Busch, J Voogt, IM Siah, TA Gee… - Leukemia research, 2010 - Elsevier
BACKGROUND: Heavy water (2H2O) labelling of DNA enables the measurement of low-
level cell proliferation in vivo, using gas chromatography/pyrolysis isotope ratio mass
spectrometry (GC/P/IRMS), but the methodology has been too complex for widespread use.
Here, we report a simplified method for measuring proliferation of malignant B cells in
patients with chronic lymphocytic leukemia (CLL). DESIGN AND METHODS: Patients were
labelled with 2H2O for 6 weeks; blood samples were obtained at 0, 3, and 6 weeks during …
level cell proliferation in vivo, using gas chromatography/pyrolysis isotope ratio mass
spectrometry (GC/P/IRMS), but the methodology has been too complex for widespread use.
Here, we report a simplified method for measuring proliferation of malignant B cells in
patients with chronic lymphocytic leukemia (CLL). DESIGN AND METHODS: Patients were
labelled with 2H2O for 6 weeks; blood samples were obtained at 0, 3, and 6 weeks during …