Host antibody response is a crucial defense against pathogenic infection. Here, we report a
novel technique allowing quantitative measurement of polyclonal antibody response in vivo.
This involves expression of a combinatorial library of target proteins from a candidate
pathogen on the surface of yeast Saccharomyces cerevisiae. After mixing with
serum/plasma from infected or immunized subjects, positive yeast clones were isolated via
fluorescence-activated cell sorting (FACS). Using this technique, we have studied mouse …