[HTML][HTML] Inactivation of Dnmt3b in mouse embryonic fibroblasts results in DNA hypomethylation, chromosomal instability, and spontaneous immortalization
JE Dodge, M Okano, F Dick, N Tsujimoto… - Journal of Biological …, 2005 - ASBMB
DNA hypomethylation is a hallmark of many types of solid tumors. However, it remains
elusive how DNA hypomethylation may contribute to tumorigenesis. In this study, we have
investigated how targeted disruption of the DNA methyltransferases Dnmt3a and Dnmt3b
affects the growth of mouse embryonic fibroblasts (MEFs). Our studies led to the following
observations. 1) Constitutive or conditional deletion of Dnmt3b, but not Dnmt3a, resulted in
partial loss of DNA methylation throughout the genome, suggesting that Dnmt3b, in addition …
elusive how DNA hypomethylation may contribute to tumorigenesis. In this study, we have
investigated how targeted disruption of the DNA methyltransferases Dnmt3a and Dnmt3b
affects the growth of mouse embryonic fibroblasts (MEFs). Our studies led to the following
observations. 1) Constitutive or conditional deletion of Dnmt3b, but not Dnmt3a, resulted in
partial loss of DNA methylation throughout the genome, suggesting that Dnmt3b, in addition …