A comprehensive immune cell atlas of cystic kidney disease reveals the involvement of adaptive immune cells in injury-mediated cyst progression in mice
Background Inducible disruption of cilia-related genes in adult mice results in slowly
progressive cystic disease, which can be greatly accelerated by renal injury. Methods To
identify in an unbiased manner modifier cells that may be influencing the differential rate of
cyst growth in injured versus non-injured cilia mutant kidneys at a time of similar cyst
severity, we generated a single-cell atlas of cystic kidney disease. We conducted RNA-seq
on 79,355 cells from control mice and adult-induced conditional Ift88 mice (hereafter …
progressive cystic disease, which can be greatly accelerated by renal injury. Methods To
identify in an unbiased manner modifier cells that may be influencing the differential rate of
cyst growth in injured versus non-injured cilia mutant kidneys at a time of similar cyst
severity, we generated a single-cell atlas of cystic kidney disease. We conducted RNA-seq
on 79,355 cells from control mice and adult-induced conditional Ift88 mice (hereafter …
