The epithelium lining airspaces of the human lung is maintained by regional stem cells,
including basal cells of pseudostratified airways and alveolar type 2 (AT2) pneumocytes of
the gas-exchange region. Despite effective techniques for long-term preservation of airway
basal cells, procedures for efficient preservation of functional epithelial cell types of the distal
gas-exchange region are lacking. Here we detail a method for cryobanking of epithelial cells
from either mouse or human lung tissue for preservation of their phenotypic and functional …

The epithelium lining airspaces of the human lung is maintained by regional stem cells
including basal cells of pseudostratified airways and alveolar type 2 pneumocytes (AT2) of
the alveolar gas-exchange region. Despite effective methods for long-term preservation of
airway basal cells, methods for efficient preservation of functional epithelial cell types of the
distal gas-exchange region are lacking. Here we detail a method for cryobanking of
epithelial cells from either mouse or human lung tissue for preservation of their phenotypic …