[HTML][HTML] Transient down-regulation of DNMT1 methyltransferase leads to activation and stable hypomethylation of MAGE-A1 in melanoma cells
A Loriot, E De Plaen, T Boon, C De Smet - Journal of biological chemistry, 2006 - ASBMB
MAGE-A1 belongs to a group of germ line-specific genes that rely primarily on DNA
methylation for repression in somatic tissues. In many types of tumors, the promoter of these
genes becomes demethylated and transcription becomes activated. We showed previously
that, although MZ2-MEL melanoma cells contain an active unmethylated MAGE-A1 gene,
they lack the ability to induce demethylation of newly integrated MAGE-A1 transgenes that
were methylated in vitro before transfection. In the same cells, unmethylated MAGE-A1 …
methylation for repression in somatic tissues. In many types of tumors, the promoter of these
genes becomes demethylated and transcription becomes activated. We showed previously
that, although MZ2-MEL melanoma cells contain an active unmethylated MAGE-A1 gene,
they lack the ability to induce demethylation of newly integrated MAGE-A1 transgenes that
were methylated in vitro before transfection. In the same cells, unmethylated MAGE-A1 …