Processing and assembly of the integrin, glycoprotein IIb-IIIa, in HEL cells
JP Rosa, RP McEver - Journal of Biological Chemistry, 1989 - ASBMB
We examined the biosynthetic processing and assembly of the platelet glycoprotein (GP) IIb-
IIIa complex in [35 S] methionine-labeled HEL cells, a human cell line with features of
megakaryocytes. Both GPIIb and GPIIIa were synthesized as single-chain precursors to
which high mannose N-linked oligosaccharides were added in the endoplasmic reticulum
(ER). A 5-fold excess of the major IIb precursor, preIIb, was synthesized relative to GPIIIa.
Two smaller proteins immunologically related to GPIIb were synthesized in smaller amounts …
IIIa complex in [35 S] methionine-labeled HEL cells, a human cell line with features of
megakaryocytes. Both GPIIb and GPIIIa were synthesized as single-chain precursors to
which high mannose N-linked oligosaccharides were added in the endoplasmic reticulum
(ER). A 5-fold excess of the major IIb precursor, preIIb, was synthesized relative to GPIIIa.
Two smaller proteins immunologically related to GPIIb were synthesized in smaller amounts …