[HTML][HTML] Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2
Vasopressin controls water excretion through regulation of aquaporin-2 (AQP2) trafficking in
renal collecting duct cells. Using mass spectrometry, we previously demonstrated four
phosphorylated serines (Ser 256, Ser 261, Ser 264, and Ser 269) in the carboxyl-terminal
tail of rat AQP2. Here, we used phospho-specific antibodies and protein mass spectrometry
to investigate the roles of vasopressin and cyclic AMP in the regulation of phosphorylation at
Ser 269 and addressed the role of this site in AQP2 trafficking. The V2 receptor-specific …
renal collecting duct cells. Using mass spectrometry, we previously demonstrated four
phosphorylated serines (Ser 256, Ser 261, Ser 264, and Ser 269) in the carboxyl-terminal
tail of rat AQP2. Here, we used phospho-specific antibodies and protein mass spectrometry
to investigate the roles of vasopressin and cyclic AMP in the regulation of phosphorylation at
Ser 269 and addressed the role of this site in AQP2 trafficking. The V2 receptor-specific …