Selective screening of secretory vesicle-associated proteins for autoantigens in type 1 diabetes: VAMP2 and NPY are new minor autoantigens
H Hirai, J Miura, Y Hu, H Larsson, K Larsson… - Clinical …, 2008 - Elsevier
The four major autoantigens (IA-2, IA-2β, GAD65 and insulin) of type 1 diabetes are all
associated with dense core or synaptic vesicles. This raised the possibility that other
secretory vesicle-associated proteins might be targets of the autoimmune response in type 1
diabetes. To test this hypothesis 56 proteins, two-thirds of which are associated with
secretory vesicles, were prepared by in vitro transcription/translation and screened for
autoantibodies by liquid phase radioimmunoprecipitation. Two secretory vesicle-associated …
associated with dense core or synaptic vesicles. This raised the possibility that other
secretory vesicle-associated proteins might be targets of the autoimmune response in type 1
diabetes. To test this hypothesis 56 proteins, two-thirds of which are associated with
secretory vesicles, were prepared by in vitro transcription/translation and screened for
autoantibodies by liquid phase radioimmunoprecipitation. Two secretory vesicle-associated …