Generation of murine primary colon epithelial monolayers from intestinal crypts
CK Muraleedharan, J Mierzwiak, D Feier… - JoVE (Journal of …, 2021 - jove.com
The intestinal epithelium is comprised of a single layer of cells that act as a barrier between
the gut lumen and the interior of the body. Disruption in the continuity of this barrier can
result in inflammatory disorders such as inflammatory bowel disease. One of the limitations
in the study of intestinal epithelial biology has been the lack of primary cell culture models,
which has obliged researchers to use model cell lines derived from carcinomas. The advent
of three dimensional (3D) enteroids has given epithelial biologists a powerful tool to …
the gut lumen and the interior of the body. Disruption in the continuity of this barrier can
result in inflammatory disorders such as inflammatory bowel disease. One of the limitations
in the study of intestinal epithelial biology has been the lack of primary cell culture models,
which has obliged researchers to use model cell lines derived from carcinomas. The advent
of three dimensional (3D) enteroids has given epithelial biologists a powerful tool to …