High-throughput sequencing of B-and T-lymphocyte antigen receptors in hematology

EH Warren, FA Matsen IV… - Blood, The Journal of the …, 2013 - ashpublications.org
EH Warren, FA Matsen IV, J Chou
Blood, The Journal of the American Society of Hematology, 2013ashpublications.org
Application of high-throughput DNA sequencing to the analysis of B-and T-lymphocyte
antigen receptors has great potential for improving the monitoring of lymphoid malignancies,
assessing immune reconstitution after hematopoietic cell transplantation, and characterizing
the composition of lymphocyte repertoires. Current technology can define the number and
frequency of immunoglobulin heavy, T-cell receptor (TCR) α, TCRβ, or TCRγ chains
expressed in a population of lymphocytes; techniques for determining the number of antigen …
Abstract
Application of high-throughput DNA sequencing to the analysis of B- and T-lymphocyte antigen receptors has great potential for improving the monitoring of lymphoid malignancies, assessing immune reconstitution after hematopoietic cell transplantation, and characterizing the composition of lymphocyte repertoires. Current technology can define the number and frequency of immunoglobulin heavy, T-cell receptor (TCR)α, TCRβ, or TCRγ chains expressed in a population of lymphocytes; techniques for determining the number of antigen receptor heterodimers, such as TCRαβ pairs, expressed in the population are under development.
ashpublications.org