Inhibitory effects of anti-inflammatory drugs on interleukin-6 bioactivity
BS Kang, EY Chung, YP Yun, MK LEE… - Biological and …, 2001 - jstage.jst.go.jp
BS Kang, EY Chung, YP Yun, MK LEE, YR LEE, KS LEE, KR MIN, Y KIM
Biological and Pharmaceutical Bulletin, 2001•jstage.jst.go.jpMATERIALS AND METHODS Materials Anti-inflammatory drugs, RPMI-1640, and 3-[4, 5-
dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) were purchased from Sigma
Chemical Co., Inc.(St. Louis, MO, USA). Fetal bovine serum (FBS) and N-[2-hydroxyethyl]
piperazine-N-2-ethanesulfonic acid (HEPES) were purchased from GIBCO BRL Products
(Gaithersburg, MD, USA) and rhIL-6 from Wako Pure Chemical Ind., Ltd.(Osaka, Japan).
dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) were purchased from Sigma
Chemical Co., Inc.(St. Louis, MO, USA). Fetal bovine serum (FBS) and N-[2-hydroxyethyl]
piperazine-N-2-ethanesulfonic acid (HEPES) were purchased from GIBCO BRL Products
(Gaithersburg, MD, USA) and rhIL-6 from Wako Pure Chemical Ind., Ltd.(Osaka, Japan).
MATERIALS AND METHODS
Materials Anti-inflammatory drugs, RPMI-1640, and 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) were purchased from Sigma Chemical Co., Inc.(St. Louis, MO, USA). Fetal bovine serum (FBS) and N-[2-hydroxyethyl] piperazine-N-2-ethanesulfonic acid (HEPES) were purchased from GIBCO BRL Products (Gaithersburg, MD, USA) and rhIL-6 from Wako Pure Chemical Ind., Ltd.(Osaka, Japan).
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