Liquid chromatography–tandem mass spectrometric determination of tenofovir-diphosphate in human peripheral blood mononuclear cells
T King, L Bushman, J Kiser, PL Anderson, M Ray… - … of chromatography B, 2006 - Elsevier
To facilitate the evaluation of drug safety, virologic activity, and pharmacokinetics, an anion
exchange isolation of tenofovir-diphosphate (TFV-DP) from human peripheral blood
mononuclear cells (hPBMCs), coupled with dephosphorylation, desaltation, and detection
by LC–MS–MS was validated. hPBMCs were harvested from whole blood, lysed, and a
suspension of intracellular tenofovir moieties was produced. TFV-DP was isolated from TFV-
monophosphate (TFV-MP) and tenofovir (TFV), dephosphorylated with acid phosphatase to …
exchange isolation of tenofovir-diphosphate (TFV-DP) from human peripheral blood
mononuclear cells (hPBMCs), coupled with dephosphorylation, desaltation, and detection
by LC–MS–MS was validated. hPBMCs were harvested from whole blood, lysed, and a
suspension of intracellular tenofovir moieties was produced. TFV-DP was isolated from TFV-
monophosphate (TFV-MP) and tenofovir (TFV), dephosphorylated with acid phosphatase to …