[HTML][HTML] Nedd4-2 induces endocytosis and degradation of proteolytically cleaved epithelial Na+ channels
R Kabra, KK Knight, R Zhou, PM Snyder - Journal of Biological Chemistry, 2008 - ASBMB
As a pathway for Na+ reabsorption, the epithelial Na+ channel ENaC is critical for Na+
homeostasis and blood pressure control. Na+ transport is regulated by Nedd4-2, an E3
ubiquitin ligase that decreases ENaC expression at the cell surface. To investigate the
underlying mechanisms, we proteolytically cleaved/activated ENaC at the cell surface and
then quantitated the rate of disappearance of cleaved channels using electrophysiological
and biochemical assays. We found that cleaved ENaC channels were rapidly removed from …
homeostasis and blood pressure control. Na+ transport is regulated by Nedd4-2, an E3
ubiquitin ligase that decreases ENaC expression at the cell surface. To investigate the
underlying mechanisms, we proteolytically cleaved/activated ENaC at the cell surface and
then quantitated the rate of disappearance of cleaved channels using electrophysiological
and biochemical assays. We found that cleaved ENaC channels were rapidly removed from …