Methods for the isolation, culture, and functional characterization of sinoatrial node myocytes from adult mice
EJ Sharpe, JRS Clair, C Proenza - JoVE (Journal of Visualized …, 2016 - jove.com
Sinoatrial node myocytes (SAMs) act as the natural pacemakers of the heart, initiating each
heart beat by generating spontaneous action potentials (APs). These pacemaker APs reflect
the coordinated activity of numerous membrane currents and intracellular calcium cycling.
However the precise mechanisms that drive spontaneous pacemaker activity in SAMs
remain elusive. Acutely isolated SAMs are an essential preparation for experiments to
dissect the molecular basis of cardiac pacemaking. However, the indistinct anatomy …
heart beat by generating spontaneous action potentials (APs). These pacemaker APs reflect
the coordinated activity of numerous membrane currents and intracellular calcium cycling.
However the precise mechanisms that drive spontaneous pacemaker activity in SAMs
remain elusive. Acutely isolated SAMs are an essential preparation for experiments to
dissect the molecular basis of cardiac pacemaking. However, the indistinct anatomy …