[HTML][HTML] Different expression of adenylyl cyclase isoforms after retinoic acid induction of P19 teratocarcinoma cells
L Lipskaia, A Djiane, N Defer, J Hanoune - FEBS letters, 1997 - Elsevier
L Lipskaia, A Djiane, N Defer, J Hanoune
FEBS letters, 1997•ElsevierWe have investigated the adenylyl cyclase (AC) activity and gene expression in retinoic acid
(RA)-primed murine P19 teratocarcinoma cells, which recapitulate in vitro the first stages of
neuroectodermal formation. Here we show that the P19 stem cells possess a basal
Ca2+/CaM-stimulated AC activity, which increases about 10-fold after RA induction. The rise
of AC activity is associated with a stage-specific up-regulation of AC2, AC5 and AC8 mRNAs
and a down-regulation of AC3 mRNA. P19 cells provide a powerful model to investigate the …
(RA)-primed murine P19 teratocarcinoma cells, which recapitulate in vitro the first stages of
neuroectodermal formation. Here we show that the P19 stem cells possess a basal
Ca2+/CaM-stimulated AC activity, which increases about 10-fold after RA induction. The rise
of AC activity is associated with a stage-specific up-regulation of AC2, AC5 and AC8 mRNAs
and a down-regulation of AC3 mRNA. P19 cells provide a powerful model to investigate the …
We have investigated the adenylyl cyclase (AC) activity and gene expression in retinoic acid (RA)-primed murine P19 teratocarcinoma cells, which recapitulate in vitro the first stages of neuroectodermal formation. Here we show that the P19 stem cells possess a basal Ca2+/CaM-stimulated AC activity, which increases about 10-fold after RA induction. The rise of AC activity is associated with a stage-specific up-regulation of AC2, AC5 and AC8 mRNAs and a down-regulation of AC3 mRNA. P19 cells provide a powerful model to investigate the role and specific regulation of AC isoforms during neuronal differentiation.
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