[HTML][HTML] Foam cell formation containing lipid droplets enriched with free cholesterol by hyperlipidemic serum
M Mori, H Itabe, Y Higashi, Y Fujimoto, M Shiomi… - Journal of lipid …, 2001 - ASBMB
A monoclonal antibody, ASH1a/256C (256C), which binds to atherosclerotic lesions in
Watanabe heritable hyperlipidemic rabbit (WHHL) aorta in vivo, recognizes complex
structures of phosphatidylcholine mixed with neutral lipids. In the present study, a cell culture
system is described in which foam cells express 256C-positive lipid droplets. J774. 1
macrophages were incubated in the presence of a small volume of WHHL serum for 24 h to
produce foam cells, which were then incubated without the WHHL serum for 3 days. Oil red …
Watanabe heritable hyperlipidemic rabbit (WHHL) aorta in vivo, recognizes complex
structures of phosphatidylcholine mixed with neutral lipids. In the present study, a cell culture
system is described in which foam cells express 256C-positive lipid droplets. J774. 1
macrophages were incubated in the presence of a small volume of WHHL serum for 24 h to
produce foam cells, which were then incubated without the WHHL serum for 3 days. Oil red …