The fluorescent cholesterol probe filipin has been used in this study to histochemically
examine the morphology and cholesterol composition of intra-and extracellular
Sudanophilie lipid deposits which accumulate in human atherosclerotic lesions. Because
filipin reacts with unesterified but not esterified cholesterol, detection of cholesteryl esters
was carried out by first extracting native unesterified cholesterol with ethanol, and then
enzymatically converting esterified to unesterified cholesterol before filipin staining. The size …