[HTML][HTML] Transcriptional networks are associated with resistance to Mycobacterium tuberculosis infection
C Seshadri, N Sedaghat, M Campo, G Peterson… - PloS one, 2017 - journals.plos.org
C Seshadri, N Sedaghat, M Campo, G Peterson, RD Wells, GS Olson, DR Sherman…
PloS one, 2017•journals.plos.orgRationale Understanding mechanisms of resistance to M. tuberculosis (M. tb) infection in
humans could identify novel therapeutic strategies as it has for other infectious diseases,
such as HIV. Objectives To compare the early transcriptional response of M. tb-infected
monocytes between Ugandan household contacts of tuberculosis patients who demonstrate
clinical resistance to M. tb infection (cases) and matched controls with latent tuberculosis
infection. Methods Cases (n= 10) and controls (n= 18) were selected from a long-term …
humans could identify novel therapeutic strategies as it has for other infectious diseases,
such as HIV. Objectives To compare the early transcriptional response of M. tb-infected
monocytes between Ugandan household contacts of tuberculosis patients who demonstrate
clinical resistance to M. tb infection (cases) and matched controls with latent tuberculosis
infection. Methods Cases (n= 10) and controls (n= 18) were selected from a long-term …
Rationale
Understanding mechanisms of resistance to M. tuberculosis (M.tb) infection in humans could identify novel therapeutic strategies as it has for other infectious diseases, such as HIV.
Objectives
To compare the early transcriptional response of M.tb-infected monocytes between Ugandan household contacts of tuberculosis patients who demonstrate clinical resistance to M.tb infection (cases) and matched controls with latent tuberculosis infection.
Methods
Cases (n = 10) and controls (n = 18) were selected from a long-term household contact study in which cases did not convert their tuberculin skin test (TST) or develop tuberculosis over two years of follow up. We obtained genome-wide transcriptional profiles of M.tb-infected peripheral blood monocytes and used Gene Set Enrichment Analysis and interaction networks to identify cellular processes associated with resistance to clinical M.tb infection.
Measurements and main results
We discovered gene sets associated with histone deacetylases that were differentially expressed when comparing resistant and susceptible subjects. We used small molecule inhibitors to demonstrate that histone deacetylase function is important for the pro-inflammatory response to in-vitro M.tb infection in human monocytes.
Conclusions
Monocytes from individuals who appear to resist clinical M.tb infection differentially activate pathways controlled by histone deacetylase in response to in-vitro M.tb infection when compared to those who are susceptible and develop latent tuberculosis. These data identify a potential cellular mechanism underlying the clinical phenomenon of resistance to M.tb infection despite known exposure to an infectious contact.
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