Low access resistance perforated patch recordings using amphotericin B
J Rae, K Cooper, P Gates, M Watsky - Journal of neuroscience methods, 1991 - Elsevier
J Rae, K Cooper, P Gates, M Watsky
Journal of neuroscience methods, 1991•ElsevierWe present experimental procedures describing the creation of perforated patches by use of
amphotericin B. In 13 different cellular preparations, access resistances below 10 MΩ were
achieved and with blunt electrode tips, access resistances of 3–4 MΩ were possible. In
addition to using the techniques to measure whole cell currents, we have used them to
measure single channel currents in a new “outside-out patch” preparation and we have
utilized them to measure the resting voltage of epithelial monolayers. We conclude that …
amphotericin B. In 13 different cellular preparations, access resistances below 10 MΩ were
achieved and with blunt electrode tips, access resistances of 3–4 MΩ were possible. In
addition to using the techniques to measure whole cell currents, we have used them to
measure single channel currents in a new “outside-out patch” preparation and we have
utilized them to measure the resting voltage of epithelial monolayers. We conclude that …
Abstract
We present experimental procedures describing the creation of perforated patches by use of amphotericin B. In 13 different cellular preparations, access resistances below 10 MΩ were achieved and with blunt electrode tips, access resistances of 3–4 MΩ were possible. In addition to using the techniques to measure whole cell currents, we have used them to measure single channel currents in a new “outside-out patch” preparation and we have utilized them to measure the resting voltage of epithelial monolayers. We conclude that these new approaches can provide a substantial increase in versatility and quality for many kinds of electrophysiological measurements.
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