[PDF][PDF] Affinity purification of recombinant trypsinogen using immobilized ecotin
Z Lengyel, G Pál, M Sahin-Tóth - Protein expression and …, 1998 - researchgate.net
FIG. 1. Interaction between ecotin and trypsinogen. Ecotin (E, 8 mg protein), bovine
trypsinogen (TG, 25 mg protein), or a mixture of the two proteins (E/TG, trypsinogen is in
excess relative to ecotin) were loaded onto a Superose 12 column (Pharmacia) equilibrated
with 50 mM Tris–HCl, pH 8.0, 0.2 M NaCl. Elution was carried out with the same buffer at a
0.5 mL/min rate.
trypsinogen (TG, 25 mg protein), or a mixture of the two proteins (E/TG, trypsinogen is in
excess relative to ecotin) were loaded onto a Superose 12 column (Pharmacia) equilibrated
with 50 mM Tris–HCl, pH 8.0, 0.2 M NaCl. Elution was carried out with the same buffer at a
0.5 mL/min rate.