[PDF][PDF] Isolation of disaturated phosphatidylcholine with osmium tetroxide
RJ Mason, J Nellenbogen, JA Clements - Journal of lipid research, 1976 - Citeseer
RJ Mason, J Nellenbogen, JA Clements
Journal of lipid research, 1976•CiteseerA simple, rapid, inexpensive method for isolat-ing disaturated phosphatidylcholine from
adult rat lung has been devised. Total lipids are reacted with osmium tetroxide dissolved in
carbon tetrachloride, and the disaturated phosphatidylcholine is isolated on a column of
neutral alumina. More than 99% of the fatty acids in the phosphatidylcholine fraction are
saturated and 94% of this material migrates as phosphatidylcholine on subsequent thinlayer
chromatography.
adult rat lung has been devised. Total lipids are reacted with osmium tetroxide dissolved in
carbon tetrachloride, and the disaturated phosphatidylcholine is isolated on a column of
neutral alumina. More than 99% of the fatty acids in the phosphatidylcholine fraction are
saturated and 94% of this material migrates as phosphatidylcholine on subsequent thinlayer
chromatography.
Summary
A simple, rapid, inexpensive method for isolat-ing disaturated phosphatidylcholine from adult rat lung has been devised. Total lipids are reacted with osmium tetroxide dissolved in carbon tetrachloride, and the disaturated phosphatidylcholine is isolated on a column of neutral alumina. More than 99% of the fatty acids in the phosphatidylcholine fraction are saturated and 94% of this material migrates as phosphatidylcholine on subsequent thinlayer chromatography.
Citeseer