Suppressors of the Ca (2+)-sensitive yeast mutant (csg2) identify genes involved in sphingolipid biosynthesis. Cloning and characterization of SCS1, a gene required …
C Zhao, T Beeler, T Dunn - Journal of Biological Chemistry, 1994 - jbc.org
Suppressor mutations in Saccharomyces cerevisiae that block Ca (2+)-induced death of
csg2 mutant cells were investigated. These mutants, called scs mutants (suppressor of Ca2+
sensitivity), fall into seven complementation groups (scs1-scs7). All mutant strains in two of
the complementation groups (scs1 and scs2) simultaneously acquire a requirement for 10
mM Ca2+, whereas wild type grow with only trace amounts of Ca2+. SCS1 was cloned by
complementation of its Ca (2+)-requiring phenotype and found to be homologous to a family …
csg2 mutant cells were investigated. These mutants, called scs mutants (suppressor of Ca2+
sensitivity), fall into seven complementation groups (scs1-scs7). All mutant strains in two of
the complementation groups (scs1 and scs2) simultaneously acquire a requirement for 10
mM Ca2+, whereas wild type grow with only trace amounts of Ca2+. SCS1 was cloned by
complementation of its Ca (2+)-requiring phenotype and found to be homologous to a family …