Ca2+/calmodulin-dependent translocation of sphingosine kinase: role in plasma membrane relocation but not activation
KW Young, JM Willets, MJ Parkinson, P Bartlett… - Cell calcium, 2003 - Elsevier
Activation of sphingosine kinase (SPHK), thereby increasing cellular levels of sphingosine 1-
phosphate (S1P), may be involved in a variety of intracellular responses including Ca2+
signaling. This study uses mammalian SPHK1a, tagged with enhanced green fluorescent
protein (eGFP), to examine whether translocation of this enzyme is linked with Ca2+-
mobilizing responses. Real-time confocal imaging of SPHK1a-eGFP in human SH-SY5Y
neuroblastoma cells visualized a relocation of the enzyme from the cytosol to the plasma …
phosphate (S1P), may be involved in a variety of intracellular responses including Ca2+
signaling. This study uses mammalian SPHK1a, tagged with enhanced green fluorescent
protein (eGFP), to examine whether translocation of this enzyme is linked with Ca2+-
mobilizing responses. Real-time confocal imaging of SPHK1a-eGFP in human SH-SY5Y
neuroblastoma cells visualized a relocation of the enzyme from the cytosol to the plasma …
