Blood and lymph node dissemination of clonal genome-intact human immunodeficiency virus 1 DNA sequences during suppressive antiretroviral therapy

HH Kuo, R Banga, GQ Lee, C Gao… - The Journal of …, 2020 - academic.oup.com
HH Kuo, R Banga, GQ Lee, C Gao, M Cavassini, JM Corpataux, JE Blackmer, S Zur Wiesch
The Journal of infectious diseases, 2020academic.oup.com
The majority of cells with latent human immunodeficiency virus 1 infection are located in
lymphoid tissues that are difficult to access. In the current study, we used single-genome
near-full-length proviral sequencing to evaluate intact and defective proviruses in blood and
lymph node CD4 T cells enriched for specific functional polarizations. We observed minor
variations between the frequencies of proviral sequences within individual CD4 T-cell
subsets and across tissue compartments. However, we noted multiple clonal clusters of …
Abstract
The majority of cells with latent human immunodeficiency virus 1 infection are located in lymphoid tissues that are difficult to access. In the current study, we used single-genome near-full-length proviral sequencing to evaluate intact and defective proviruses in blood and lymph node CD4 T cells enriched for specific functional polarizations. We observed minor variations between the frequencies of proviral sequences within individual CD4 T-cell subsets and across tissue compartments. However, we noted multiple clonal clusters of identical intact or defective proviral sequences from distinct compartments and CD4 T-cell subpopulations, suggesting frequent interchanges between viral reservoir cells in blood and tissues.
Oxford University Press