Potential role of MCP-1 in endothelial cell tight junctionopening': signaling via Rho and Rho kinase

SM Stamatovic, RF Keep, SL Kunkel… - Journal of cell …, 2003 - journals.biologists.com
SM Stamatovic, RF Keep, SL Kunkel, AV Andjelkovic
Journal of cell science, 2003journals.biologists.com
The expression of the monocyte chemoattractant protein-1 (MCP-1) receptor CCR2 by brain
endothelial cells suggests that MCP-1 may have other functions than purely driving
leukocyte migration into brain parenchyma during inflammation. This study examines one of
these potential novel roles of MCP-1 regulation of endothelial permeability using primary
cultures of mouse brain endothelial cells. MCP-1 induces reorganization of actin
cytoskeleton (stress fiber formation) and redistribution of tight junction proteins, ZO-1, ZO-2 …
The expression of the monocyte chemoattractant protein-1 (MCP-1) receptor CCR2 by brain endothelial cells suggests that MCP-1 may have other functions than purely driving leukocyte migration into brain parenchyma during inflammation. This study examines one of these potential novel roles of MCP-1 regulation of endothelial permeability using primary cultures of mouse brain endothelial cells. MCP-1 induces reorganization of actin cytoskeleton (stress fiber formation) and redistribution of tight junction proteins, ZO-1, ZO-2 occludin and claudin-5, from the Triton X-100-soluble to the Triton X-100-insoluble fractions. These morphological changes are associated with a decrease in transendothelial electrical membrane resistance and an increase in [14C]inulin permeability. MCP-1 did not induce these events in brain endothelial cells prepared from mice genotype CCR2–/–. The Rho kinase inhibitor Y27632 and inhibition of Rho (C3 exoenzyme, and dominant negative mutant of Rho, RhoT19N) prevented MCP-1-induced stress fiber assembly, reorganization of tight junction proteins and alterations in endothelial permeability. In all, this suggests that a small GTPase Rho and Rho kinase have a pivotal role in MCP-1-induced junction disarrangement. These data are the first to strongly suggest that MCP-1, via CCR2 present on brain endothelial cells, contributes to increased brain endothelial permeability.
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