Selective targeting of mutant adenomatous polyposis coli (APC) in colorectal cancer
Science translational medicine, 2016•science.org
Mutations in the adenomatous polyposis coli (APC) gene are common in colorectal cancer
(CRC), and more than 90% of those mutations generate stable truncated gene products. We
describe a chemical screen using normal human colonic epithelial cells (HCECs) and a
series of oncogenically progressed HCECs containing a truncated APC protein. With this
screen, we identified a small molecule, TASIN-1 (truncated APC selective inhibitor–1), that
specifically kills cells with APC truncations but spares normal and cancer cells with wild-type …
(CRC), and more than 90% of those mutations generate stable truncated gene products. We
describe a chemical screen using normal human colonic epithelial cells (HCECs) and a
series of oncogenically progressed HCECs containing a truncated APC protein. With this
screen, we identified a small molecule, TASIN-1 (truncated APC selective inhibitor–1), that
specifically kills cells with APC truncations but spares normal and cancer cells with wild-type …
Mutations in the adenomatous polyposis coli (APC) gene are common in colorectal cancer (CRC), and more than 90% of those mutations generate stable truncated gene products. We describe a chemical screen using normal human colonic epithelial cells (HCECs) and a series of oncogenically progressed HCECs containing a truncated APC protein. With this screen, we identified a small molecule, TASIN-1 (truncated APC selective inhibitor–1), that specifically kills cells with APC truncations but spares normal and cancer cells with wild-type APC. TASIN-1 exerts its cytotoxic effects through inhibition of cholesterol biosynthesis. In vivo administration of TASIN-1 inhibits tumor growth of CRC cells with truncated APC but not APC wild-type CRC cells in xenograft models and in a genetically engineered CRC mouse model with minimal toxicity. TASIN-1 represents a potential therapeutic strategy for prevention and intervention in CRC with mutant APC.
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