The myofibroblast is a stromal cell of the gastrointestinal (GI) tract that has been gaining
considerable attention for its critical role in many GI functions. While several myofibroblast
cell lines are commercially available to study these cells in vitro, research results from a cell
line exposed to experimental cell culture conditions have inherent limitations due to the
overly reductionist nature of the work. Use of primary myofibroblasts offers a great
advantage in terms of confirming experimental findings identified in a cell line. Isolation of …