uc. 77-downregulation promotes colorectal cancer cell proliferation by inhibiting FBXW8-mediated CDK4 protein degradation
Z Zheng, D Hong, X Zhang, Y Chang, N Sun… - Frontiers in …, 2021 - frontiersin.org
Z Zheng, D Hong, X Zhang, Y Chang, N Sun, Z Lin, H Li, S Huang, R Zhang, Q Xie, H Huang…
Frontiers in oncology, 2021•frontiersin.orgTranscribed ultraconserved regions (T-UCRs) are a new type of long non-coding RNA, and
the UCR has 481 segments longer than 200 base pairs that are 100% conserved between
humans, rats, and mice. T-UCRs involved in colorectal cancer (CRC) have not been studied
in detail. We performed T-UCR microarray analysis and found that uc. 77-was significantly
downregulated in CRC tissues and cell lines. Ectopic expression of uc. 77-significantly
inhibited the proliferation of CRC cells in vitro and the growth of xenograft tumors in nude …
the UCR has 481 segments longer than 200 base pairs that are 100% conserved between
humans, rats, and mice. T-UCRs involved in colorectal cancer (CRC) have not been studied
in detail. We performed T-UCR microarray analysis and found that uc. 77-was significantly
downregulated in CRC tissues and cell lines. Ectopic expression of uc. 77-significantly
inhibited the proliferation of CRC cells in vitro and the growth of xenograft tumors in nude …
Transcribed ultraconserved regions (T-UCRs) are a new type of long non-coding RNA, and the UCR has 481 segments longer than 200 base pairs that are 100% conserved between humans, rats, and mice. T-UCRs involved in colorectal cancer (CRC) have not been studied in detail. We performed T-UCR microarray analysis and found that uc.77- was significantly downregulated in CRC tissues and cell lines. Ectopic expression of uc.77- significantly inhibited the proliferation of CRC cells in vitro and the growth of xenograft tumors in nude mice in vivo. Mechanistic studies showed that uc.77- competed with FBXW8 mRNA for binding to microRNA (miR)-4676-5p through a competing endogenous RNA mechanism and inhibited the proliferation of CRC cells by negatively regulating CDK4. The present findings highlight the role of the uc.77-/miR-4676-5p/FBXW8 axis in CRC and identify uc.77- as a potential novel target for the treatment of CRC.
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