[HTML][HTML] Regulation of pri-miRNA processing by a long noncoding RNA transcribed from an ultraconserved region
Molecular cell, 2014•cell.com
Noncoding RNAs (ncRNAs) control cellular programs by affecting protein-coding genes, but
evidence increasingly points to their involvement in a network of ncRNA-ncRNA interactions.
Here, we show that a long ncRNA, Uc. 283+ A, controls pri-miRNA processing. Regulation
requires complementarity between the lower stem region of the pri-miR-195 transcript and
an ultraconserved sequence in Uc. 283+ A, which prevents pri-miRNA cleavage by Drosha.
Mutation of the site in either RNA molecule uncouples regulation in vivo and in vitro. We …
evidence increasingly points to their involvement in a network of ncRNA-ncRNA interactions.
Here, we show that a long ncRNA, Uc. 283+ A, controls pri-miRNA processing. Regulation
requires complementarity between the lower stem region of the pri-miR-195 transcript and
an ultraconserved sequence in Uc. 283+ A, which prevents pri-miRNA cleavage by Drosha.
Mutation of the site in either RNA molecule uncouples regulation in vivo and in vitro. We …
Summary
Noncoding RNAs (ncRNAs) control cellular programs by affecting protein-coding genes, but evidence increasingly points to their involvement in a network of ncRNA-ncRNA interactions. Here, we show that a long ncRNA, Uc.283+A, controls pri-miRNA processing. Regulation requires complementarity between the lower stem region of the pri-miR-195 transcript and an ultraconserved sequence in Uc.283+A, which prevents pri-miRNA cleavage by Drosha. Mutation of the site in either RNA molecule uncouples regulation in vivo and in vitro. We propose a model in which lower-stem strand invasion by Uc.283+A impairs microprocessor recognition and efficient pri-miRNA cropping. In addition to identifying a case of RNA-directed regulation of miRNA biogenesis, our study reveals regulatory networks involving different ncRNA classes of importance in cancer.
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