[HTML][HTML] Native mutant huntingtin in human brain: evidence for prevalence of full-length monomer
E Sapp, A Valencia, X Li, N Aronin, KB Kegel… - Journal of Biological …, 2012 - ASBMB
Huntington disease (HD) is caused by polyglutamine expansion in the N terminus of
huntingtin (htt). Analysis of human postmortem brain lysates by SDS-PAGE and Western blot
reveals htt as full-length and fragmented. Here we used Blue Native PAGE (BNP) and
Western blots to study native htt in human postmortem brain. Antisera against htt detected a
single band broadly migrating at 575–850 kDa in control brain and at 650–885 kDa in
heterozygous and Venezuelan homozygous HD brains. Anti-polyglutamine antisera …
huntingtin (htt). Analysis of human postmortem brain lysates by SDS-PAGE and Western blot
reveals htt as full-length and fragmented. Here we used Blue Native PAGE (BNP) and
Western blots to study native htt in human postmortem brain. Antisera against htt detected a
single band broadly migrating at 575–850 kDa in control brain and at 650–885 kDa in
heterozygous and Venezuelan homozygous HD brains. Anti-polyglutamine antisera …