Diffuse Intrinsic Pontine Glioma (DIPG) is a childhood brainstem tumor that carries a
universally fatal prognosis. Because surgical resection is not a viable treatment strategy and
biopsy is not routinely performed, the availability of patient samples for research is limited.
Consequently, efforts to study this disease have been challenged by a paucity of faithful
disease models. To address this need, we describe here a protocol for the rapid processing
of post-mortem autopsy tissue samples in order to generate durable patient-derived cell …