Sensitive detection of metallothioneins-1,-2 and-3 in tissue homogenates by immunoblotting: a method for enhanced membrane transfer and retention
CA Mizzen, NJ Cartel, WH Yu, PE Fraser… - Journal of biochemical …, 1996 - Elsevier
Standard immunoblotting procedures were unable to detect metallothioneins-1 and 2 (MT-1,
MT-2) and metallothionein-3 (MT-3)/growth inhibitory factor (GIF) in unfractionated brain
homogenates. We have developed a novel process which involves the inclusion of 2 mM
CaCl2 in electrophoretic transfer buffers and glutaraldehyde fixation following transfer to
either nitrocellulose or polyvinylidene difluoride (PVDF) membranes. Using commercial MT
antibodies and a specific MT-3 polyclonal antibody raised in our laboratory, we have been …
MT-2) and metallothionein-3 (MT-3)/growth inhibitory factor (GIF) in unfractionated brain
homogenates. We have developed a novel process which involves the inclusion of 2 mM
CaCl2 in electrophoretic transfer buffers and glutaraldehyde fixation following transfer to
either nitrocellulose or polyvinylidene difluoride (PVDF) membranes. Using commercial MT
antibodies and a specific MT-3 polyclonal antibody raised in our laboratory, we have been …