Generation of high-titer pseudotyped retroviral vectors with very broad host range
JK Yee, T Friedmann, JC Burns - Methods in cell biology, 1994 - Elsevier
Publisher Summary This chapter discusses the procedure to generate high-titer
pseudotyped retroviral vectors and to use these vectors to infect nonmammalian cells. The
chapter presents a retroviral construct, plasmid pLZRNL. Plasmid pLZRNL contains the
gene encoding Escherichia coli β-galactosidase (LacZ) controlled by the 5′ long terminal
repeat (LTR) of Moloney murine sarcoma virus followed by the gene encoding neomycin
phosphotransferase (Neo) controlled by the promoter of Rous sarcoma virus (RSV). The …
pseudotyped retroviral vectors and to use these vectors to infect nonmammalian cells. The
chapter presents a retroviral construct, plasmid pLZRNL. Plasmid pLZRNL contains the
gene encoding Escherichia coli β-galactosidase (LacZ) controlled by the 5′ long terminal
repeat (LTR) of Moloney murine sarcoma virus followed by the gene encoding neomycin
phosphotransferase (Neo) controlled by the promoter of Rous sarcoma virus (RSV). The …