Gas chromatographic–mass spectrometric procedures for determination of the catechol-O-methyltransferase (COMT) activity and for detection of unstable catecholic …
HH Maurer, J Bickeboeller-Friedrich… - Journal of Chromatography …, 2000 - Elsevier
A procedure is presented for determination of the catechol-O-methyltransferase (COMT)
activity in liver cytosolic preparations using 3, 4-dihydroxyphenethylamine as substrate and
by quantifying the product 3-methoxy-4-hydroxyphenethylamine (3-MHP). For quantification
of 3-MHP in liver cytosolic preparations a gas chromatographic–mass spectrometric
procedure after liquid–liquid extraction and acetylation was established and validated. The
intra-and inter-day accuracy and precision were better than 15% and 20%, respectively …
activity in liver cytosolic preparations using 3, 4-dihydroxyphenethylamine as substrate and
by quantifying the product 3-methoxy-4-hydroxyphenethylamine (3-MHP). For quantification
of 3-MHP in liver cytosolic preparations a gas chromatographic–mass spectrometric
procedure after liquid–liquid extraction and acetylation was established and validated. The
intra-and inter-day accuracy and precision were better than 15% and 20%, respectively …