[HTML][HTML] The-4 phenylalanine is required for substrate ubiquitination catalyzed by HECT ubiquitin ligases
C Salvat, G Wang, A Dastur, N Lyon… - Journal of biological …, 2004 - ASBMB
The reaction cycle of HECT domain ubiquitin ligases consists of three steps: 1) binding of an
E2 protein, 2) transfer of ubiquitin from E2 to the HECT domain, and 3) transfer of ubiquitin to
the substrate. We report the identification of a determinant that is specifically required for the
last step of this cycle, a phenylalanine residue located four amino acids from the C terminus
of most HECT domains, referred to here as the-4F. Alteration of this residue in human E6AP
and Saccharomyces cerevisae Rsp5p did not affect ubiquitin-thioester formation, but …
E2 protein, 2) transfer of ubiquitin from E2 to the HECT domain, and 3) transfer of ubiquitin to
the substrate. We report the identification of a determinant that is specifically required for the
last step of this cycle, a phenylalanine residue located four amino acids from the C terminus
of most HECT domains, referred to here as the-4F. Alteration of this residue in human E6AP
and Saccharomyces cerevisae Rsp5p did not affect ubiquitin-thioester formation, but …