Different epidermal growth factor (EGF) receptor ligands show distinct kinetics and biased or partial agonism for homodimer and heterodimer formation
JL Macdonald-Obermann, LJ Pike - Journal of Biological Chemistry, 2014 - jbc.org
The EGF receptor has seven different cognate ligands. Previous work has shown that these
different ligands are capable of inducing different biological effects, even in the same cell. To
begin to understand the molecular basis for this variation, we used luciferase fragment
complementation to measure ligand-induced dimer formation and radioligand binding to
study the effect of the ligands on subunit-subunit interactions in EGF receptor (EGFR)
homodimers and EGFR/ErbB2 heterodimers. In luciferase fragment complementation …
different ligands are capable of inducing different biological effects, even in the same cell. To
begin to understand the molecular basis for this variation, we used luciferase fragment
complementation to measure ligand-induced dimer formation and radioligand binding to
study the effect of the ligands on subunit-subunit interactions in EGF receptor (EGFR)
homodimers and EGFR/ErbB2 heterodimers. In luciferase fragment complementation …
