[PDF][PDF] A regulatory B cell subset with a unique CD1dhiCD5+ phenotype controls T cell-dependent inflammatory responses

K Yanaba, JD Bouaziz, KM Haas, JC Poe, M Fujimoto… - Immunity, 2008 - cell.com
K Yanaba, JD Bouaziz, KM Haas, JC Poe, M Fujimoto, TF Tedder
Immunity, 2008cell.com
B cells mediate multiple functions that influence immune and inflammatory responses. In this
study, T cell-mediated inflammation was exaggerated in CD19-deficient (Cd19−/−) mice and
wild-type mice depleted of CD20+ B cells, whereas inflammation was substantially reduced
in mice with hyperactive B cells as a result of CD19 overexpression (hCD19Tg). These
inflammatory responses were negatively regulated by a unique CD1d hi CD5+ B cell subset
that was absent in Cd19−/− mice, represented only 1%–2% of spleen B220+ cells in wild …
Summary
B cells mediate multiple functions that influence immune and inflammatory responses. In this study, T cell-mediated inflammation was exaggerated in CD19-deficient (Cd19−/−) mice and wild-type mice depleted of CD20+ B cells, whereas inflammation was substantially reduced in mice with hyperactive B cells as a result of CD19 overexpression (hCD19Tg). These inflammatory responses were negatively regulated by a unique CD1dhiCD5+ B cell subset that was absent in Cd19−/− mice, represented only 1%–2% of spleen B220+ cells in wild-type mice, but was expanded to ∼10% of spleen B220+ cells in hCD19Tg mice. Adoptive transfer of these CD1dhiCD5+ B cells normalized inflammation in wild-type mice depleted of CD20+ B cells and in Cd19−/− mice. Remarkably, IL-10 production was restricted to this CD1dhiCD5+ B cell subset, with IL-10 production diminished in Cd19−/− mice, yet increased in hCD19Tg mice. Thereby, CD1dhiCD5+ B cells represent a unique subset of potent regulatory B cells.
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