[HTML][HTML] Quantifying intracellular rates of glycolytic and oxidative ATP production and consumption using extracellular flux measurements
Partitioning of ATP generation between glycolysis and oxidative phosphorylation is central
to cellular bioenergetics but cumbersome to measure. We describe here how rates of ATP
generation by each pathway can be calculated from simultaneous measurements of
extracellular acidification and oxygen consumption. We update theoretical maximum ATP
yields by mitochondria and cells catabolizing different substrates. Mitochondrial P/O ratios
(mol of ATP generated per mol of [O] consumed) are 2.73 for oxidation of pyruvate plus …
to cellular bioenergetics but cumbersome to measure. We describe here how rates of ATP
generation by each pathway can be calculated from simultaneous measurements of
extracellular acidification and oxygen consumption. We update theoretical maximum ATP
yields by mitochondria and cells catabolizing different substrates. Mitochondrial P/O ratios
(mol of ATP generated per mol of [O] consumed) are 2.73 for oxidation of pyruvate plus …