Mitochondrial respiratory chain disease is caused by a wide range of individually rare
genetic disorders that impair cellular energy metabolism. While fluorescence microscopy
analysis of nematodes fed MitoTracker Green (MTG) and tetramethylrhodamine ethyl ester
(TMRE) can reliably quantify relative mitochondrial density and membrane potential,
respectively, in C. elegans models of mitochondrial dysfunction, it is a tedious process with
limitations in the number and age of animals that can be studied. A novel, large particle, flow …