[HTML][HTML] Cellular immune responses in amniotic fluid of women with preterm prelabor rupture of membranes
Journal of perinatal medicine, 2020•degruyter.com
Background Preterm birth is the leading cause of perinatal morbidity and mortality. Preterm
prelabor rupture of membranes (pPROM) occurs in 30% of preterm births; thus, this
complication is a major contributor to maternal and neonatal morbidity. However, the cellular
immune responses in amniotic fluid of women with pPROM have not been investigated.
Methods Amniotic fluid samples were obtained from women with pPROM and a positive (n=
7) or negative (n= 10) microbiological culture. Flow cytometry was performed to evaluate the …
prelabor rupture of membranes (pPROM) occurs in 30% of preterm births; thus, this
complication is a major contributor to maternal and neonatal morbidity. However, the cellular
immune responses in amniotic fluid of women with pPROM have not been investigated.
Methods Amniotic fluid samples were obtained from women with pPROM and a positive (n=
7) or negative (n= 10) microbiological culture. Flow cytometry was performed to evaluate the …
Background
Preterm birth is the leading cause of perinatal morbidity and mortality. Preterm prelabor rupture of membranes (pPROM) occurs in 30% of preterm births; thus, this complication is a major contributor to maternal and neonatal morbidity. However, the cellular immune responses in amniotic fluid of women with pPROM have not been investigated.
Methods
Amniotic fluid samples were obtained from women with pPROM and a positive (n = 7) or negative (n = 10) microbiological culture. Flow cytometry was performed to evaluate the phenotype and number of amniotic fluid leukocytes. The correlation between amniotic fluid immune cells and an interleukin-6 (IL-6) concentration or a white blood cell (WBC) count in amniotic fluid was calculated.
Results
Women with pPROM and a positive amniotic fluid culture had (1) a greater number of total leukocytes in amniotic fluid, including neutrophils and monocytes/macrophages and (2) an increased number of total T cells in amniotic fluid, namely CD4+ T cells and CD8+ T cells, but not B cells. The numbers of neutrophils and monocytes/macrophages were positively correlated with IL-6 concentrations and WBC counts in amniotic fluid of women with pPROM.
Conclusion
Women with pPROM and a positive amniotic fluid culture exhibit a more severe cellular immune response than those with a negative culture, which is associated with well-known markers of intra-amniotic inflammation.
De Gruyter