Correlation of the quantitative level of MGMT promoter methylation and overall survival in primary diagnosed glioblastomas using the quantitative MethyQESD method
C von Rosenstiel, B Wiestler, B Haller… - Journal of clinical …, 2020 - jcp.bmj.com
Journal of clinical pathology, 2020•jcp.bmj.com
Aims O (6)-methylguanine-DNA-methyltransferase (MGMT) promoter methylation is a high
predictive factor for therapy results of temozolomide in patients with glioma. The objective of
this work was to analyse the impact of MGMT promoter methylation in patients with primary
diagnosed glioblastoma (GBM) relating to survival using a quantitative method (methylation
quantification of endonuclease-resistant DNA, MethyQESD) by verifying a cut-off point for
MGMT methylation provided by the literature (
predictive factor for therapy results of temozolomide in patients with glioma. The objective of
this work was to analyse the impact of MGMT promoter methylation in patients with primary
diagnosed glioblastoma (GBM) relating to survival using a quantitative method (methylation
quantification of endonuclease-resistant DNA, MethyQESD) by verifying a cut-off point for
MGMT methylation provided by the literature (
Aims
O(6)-methylguanine-DNA-methyltransferase (MGMT) promoter methylation is a high predictive factor for therapy results of temozolomide in patients with glioma. The objective of this work was to analyse the impact of MGMT promoter methylation in patients with primary diagnosed glioblastoma (GBM) relating to survival using a quantitative method (methylation quantification of endonuclease-resistant DNA, MethyQESD) by verifying a cut-off point for MGMT methylation provided by the literature (
Methods
67 patients aged 70 years or younger, operated between January 2013 and December 2015, with newly diagnosed IDH wild-type GBM and clinical follow-up were retrospectively investigated in this study. A known MGMT promoter methylation status was the inclusion criteria.
Results
Median overall survival (OS) was 16.9 months. Patients who had a methylated MGMT promoter region of ≥10% had an improved OS compared with patients with a methylated promoter region of <10% (p=0.002). Optimal cut-off point for MGMT promoter methylation was 11.7% (p=0.012).
Conclusion
The results confirm that the quantitative level of MGMT promoter methylation is a positive prognostic factor in newly diagnosed patients with GBM. The cut-off provided by the literature (
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